APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Understand the general rule of cell identification. DR. ANWAR H. SIDDIQUI. Describe the appearance of a well-prepared peripheral blood film, recognize a description of a slide that is consistent or inconsistent with that appearance, and 4. A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Now customize the name of a clipboard to store your clips. 3.Film should not be too thin or too thick . The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. No area contains large amounts of broken cells or precipitated stain. A good wedge smear should cover half to three fourths of the slide, should show gradual transition from a thick to a thin end with a feathered edge. In patients with anemia, the Making a great blood smear (or blood film as some call it) is an important skill for any veterinary professional, even if your hospital does not require differentials to be done on a regular basis.It’s a great way to impress your colleagues! Has both thick and thin areas with gradual transition. A polychrome stain, contains methylene blue and eosine dye dissolved in methyl alcohol. Peripheral blood smear of a 38-year-old female with long-standing Crohn’s disease (CD) and development of microcytic anemia. Prolonged storage of anticoagulated blood specimen, Delay in spreading the blood drop, Dirty and poor quality glass slide, Inappropriate size of blood drop, Improper angle of the pusher slide, Improper pressure/speed, High Humidity. An IUD does not interfere with the ability to get a good sample. State the components and function of a Wright's Stain. Decrease in the amount of thrombocytes seen in acute leukemia , radiation therapy. Erythrocyte Morphology, Platelet evaluation, Quantitative White Blood Cells. In peripheral blood film (PBF), neutrophils showed characteristic C-shaped, fetus-like nuclei, elongated nucleoplasm, and ring-shaped nuclei. 3. Describe the techniques for making peripheral blood films. Attention to detail in slide preparation, staining and standard criteria for cell estimates, morphology and differential reporting enhance the value of Peripheral Blood Smear Test Procedure The blood smear test is a simple procedure in which your health care provider draws a blood … Characteristics of A Good Smear 1. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… List the causes of a poor blood smear preparation. ⇒ Flame the slide and place it on the disinfected table. Examining the monolayer of a well-prepared and properly stained blood smear allows veterinary technicians to assess erythrocyte (RBC), leukocyte (WBC) and platelet morphology. Clinical Case Study Fatigued: A Case on Blood Download Case Study with Worksheet Harold is a 67-year-old male who has had regular physical exams, is a non-smoker, and who has been in good health for most of his life. [1] Lymphocytes include natural killer cells (which function in cell-mediated, cytotoxic innate immunity), T cells (for cell-mediated, cytotoxic adaptive immunity), and B cells (for humoral, antibody-driven adaptive immunity). Two push-type peripheral blood smears suitable for characterization of cellular blood elements. 1. It is often done as part of or along with a complete blood count (CBC). 2.Smear should be tongue shaped . The manual peripheral blood smear Whilst the manual differential count is generally considered to be superior to an analyser generated count for pathologi-cal samples, this only holds true under very strict conditions. Peripheral Microcytic when the size is smaller than normal RBC and these are less than 6 µm. See our User Agreement and Privacy Policy. Describe different erythrocyte inclusions, their etiology and their clinical significance. However, microscopic evaluation of peripheral blood smears affords clinicians additional data that may be used to guide diagnosis and treatment. blood smear. Describe the blood specimen and procedure for preparing a blood smear. There are many reasons why your doctor may order a blood smear. Peripheral blood film or Peripheral blood smear or Peripheral smear examination forms an important and basic lab investigation for diagnosing a number of hematological (blood) disorders. Normochromic when the color is normal. 11. State the appropriate sample used for preparing a peripheral blood smear. BLOOD SMEAR BASICS JENNIFER A. NEEL, DVM, DACVP (CLINICAL) ASSOCIATE PROFESSOR, CLINICAL PATHOLOGY NC STATE COLLEGE OF VETERINARY MEDICINE RALEIGH, NC, … 4. Characteristics of good smear : 1.Surface should be even and uniform ,margins should not extend to the side of the slide. A blood smear is considered abnormal when there’s an abnormality in the size, shape, color, or number of cells in your blood. Bacteria, Babesia or Malaria, Stain Precipitates. Mild monocytosis (900/μL) along with the persistent lymphopenia was also noted. The blood smear demonstrated hypersegmented neutrophils (panels A,C), macrocytes (panel B), and red blood cell fragments (panels A,C). Anemia, morphology, red blood cell, poikilocytosis, peripheral smear SUMMARY The foundation of laboratory hematologic diagnosis is the complete blood count and review of the peripheral smear. Two main conditions will affect the viscosity of the blood. 30×) than in an equal area of a thin smear. Flame the slide and place it on the disinfected table. Large granular lymphocytes noted, a representation of natural killer cells or cytotoxic T lymphocytes. A blood smear is a blood test that gives information about the number and shape of blood cells. Normal, mature RBCs are biconcave, disc-shaped, anuclear cells measuring approximately 7-8 microns in diameter on a peripheral blood smear with an internal volume of 80-100 femtoliters (fL). You can change your ad preferences anytime. Parts of the Compound Microscope Tube It is cylindrical tube through which the light from an illuminated object traverses enroute its formation of an image. A. PROCEDURE FOR PREPARING AN IDEAL SMEAR ⇒ Take a Clean, Grease free microscopic glass slide and mark the smear area on the underside of the slide with a glass marking pencil. Peripheral Blood Smear 1. 2. 3. List the characteristics of a good blood smear Good Blood Smear - Margin free with distinct lateral edge, Smooth Appearance, Placed centrally on the slide, Has uniform distribution of leukocytes, Whole drop of blood is picked up and spread, Has gradual transition from thick to thin ,When held up to light has a rainbow appearance. LIst the components of peripheral blood smear examination. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Improper timing of staining or buffering, Deteriorated reagent. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Cell Size (immature cells are larger), Nuclear to cytoplasmic ratio (relative and absoluate size of the nucleus decreases as a result of nuclear maturation) , RBC's (nucleus and other organelles disappear), Nucleus (Young nucleus is reddish and bcome bluer as cell ages, chromatin is fine and lacy and becomes coarse, clumped and condensed as cells mature), Cytoplasm (Granulocyte matures from non granulated to specific granules which differs in dye affinity, granules increase in number). 1. Looks like you’ve clipped this slide to already. Serum folate and vitamin B 12 were normal, but plasma homocysteine was elevated (>100.0 μmol/L), methionine decreased (10 μmol/L), and methylmalonic acid normal, indicating a specific homocysteine to methionine conversion defect. List the characteristics of a good blood smear. You more relevant ads cytotoxic T lymphocytes used to guide diagnosis and treatment to: 1 their significance... 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